Job offersmore »
- Technical Sales Representative Trainee - Ancaster, Ontario
- International Account Manager City Farming - Horticulture LED Solutions
- CEO for a leading Agri-Business working on an international basis
- Greenhouse Operations Lead - Alberta, Canada
- Commercial Head Grower - Newark, NJ (USA)
- IPM & Pollination Specialist (ornamentals) - Western Europe
- Regional Sales Manager - USA
- General Manager Operations - Australia
- International Account manager Horticulture LED Solutions - Netherlands
- Plant Specialist Horticulture Northern Europe
Top 5 - yesterday
Top 5 - last week
- "We are building a global brand, it's not just about selling apples"
- "Our Kissabel apples go beyond offering a new variety"
- “An auction is a good way to sell large volumes in a short time”
- Accu-Label customizes labeling solutions and introduces new tray labeler
- “Our Edipeel product keeps ripe avocados ripe for twice as long”
Top 5 - last month
- Dominican Republic evaluates damage after Hurricane Maria
- India: New packaging technologies for South America
- California company celebrates 10 years of attending PMA’s Fresh Summit
- "We hope the arrival of Spanish lemons will reactivate the market in Europe"
- Peru: French company is interested in dried organic produce
Exchange ratesmore »
QUAFETY project shows the prevalence and biodiversity of bacterial pathogens in produceScientists of the Department of Food Science and Technology at Agricultural University of Athens (Greece), within the QUAFETY European project (2011-2014), determined the prevalence of Listeria monocytogenes and Escherichia coli O157:H7 in fresh-cut rocket, cucumbers and strawberries.
The detection of both pathogens was based on the respective ISO methods with a parallel study for the comparison of two chromogenic culture media: ALOA and RAPID'L. mono in the former case, CT - SMAC and Fluorocult in the latter.
Confirmation of the suspect colonies was performed by hemolysis, rhamnose and xylose fermentation as well as specific PCR for L. monocytogenes; Latex Test and specific PCR for E. coli O157:H7.
The results of biochemical tests and molecular analyses showed that the prevalence of L. monocytogenes was 7% in rocket, 6% in cucumbers and 3.8% in strawberries, while prevalence of E. coli O157: H7 was 7% in rocket, 3% in cucumbers and 3.8% in strawberries. Furthermore, it was concluded that parallel use of more than one chromogenic media is necessary for accurate estimation of L. monocytogenes and E. coli O157:H7 prevalence.
The same scientists have also assessed the biodiversity of 22 L. monocytogenes and 12 Escherichia coli Ο157:Η7 strains isolated from rocket, cucumber and strawberry samples, as well as the detection of virulence - associated genes.
Genotypic diversity of both pathogens was assessed by RAPD - PCR using M13, UBC155 and HLWL85 as primers and by rep - PCR using (GTG)5 as primer. Results showed that all L. monocytogenes strains belonged to 4b serotype. Many virulence associated genes such as plcB, plcA, actA, hlyA regarding L. monocytogenes and stx2, eae and some of their variants regarding E. coli O157:H7 have been detected. However, none of the isolates contained all necessary genes required for pathogenicity, as detected by published specific - PCR protocols, most probably due to variations in the primer hybridization sequences.
Source: Hadjilouka A., Madjourani K.S., Katsarou A., Koubou V., Paramithiotis S., Mataragas M., Drosinos, E.H., Laboratory of Food Quality Control and Hygiene, Department of Food Science and Technology, Agricultural University of Athens, Greece. www.quafety.eu
Receive the daily newsletter in your email for free | Click here